Cytotoxic Effects of Coated Gold Nanoparticles on PC12 Cancer Cell

  • Hadi Zare Marzouni Department of Immunology, faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  • Fazel Tarkhan Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran
  • Amir Aidun 1-National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran 2-Tissues and Biomaterial Research Group (TBRG), Universal Scientific Education and Research Network (USERN), Tehran, Iran
  • Kiana Shahzamani Hepatitis research Center, Lorestan University of Medical Science , Khorramabad, Iran
  • Hamid Reza Jahan Tigh Department of Immunology, faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  • Sareh Malekshahian Department of biotechnology, Islamic Azad University, Urmia branch, Urmia, Iran
  • Hamed Esmaeil Lashgarian Hepatitis research Center, Lorestan University of Medical Science , Khorramabad, Iran
DOI: https://doi.org/10.31661/gmj.v7i0.1110
Keywords: Gold Nanoparticle, Cancer, Apoptosis, ROS, LDH

Abstract

Background: The use of gold nanoparticles in medicine and especially in cancer treatment has been of interest to researchers. The effectiveness of this nanoparticle on cells significantly depends on the amount of its entry into the cells. This study was performed to compare the rate and mechanism of effect of gold nanoparticles coated with different amino acid on PC12 cancer cell line.Materials and Methods: The PC12 cells line were exposed to various concentrations of amino acid coated and uncoated gold nanoparticles (0.5, 2.5 and 5 μM). Cell death rate was determined according to level of Lactate dehydrogenase (LDH) release from cells and MTT assay. In addition cell morphology and the amount of Cellular Reactive oxygen species (ROS) were studied.Results: The uncoated gold nanoparticles have shown minor effects on cellular life. Gold nanoparticles coated by tryptophan at high concentrations (2.5, 5 and 25μM) increase in cancer cells metabolic activity. Gold nanoparticles coated by Aspartate also produce the largest amount of LDH and ROS in cancer cells and therefore caused of highest rate of apoptosis.Conclusion: The results showed that the nanoparticles coated with amino acids are affected on cellular metabolism and apoptosis more than uncoated nanoparticles. Also the smallest coated nanoparticles (coated by aspartate) have the most influence and by increasing the size, this effect was reduced. [GMJ.2018;7:e1110]

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Published
2018-07-13
How to Cite
Zare Marzouni, H., Tarkhan, F., Aidun, A., Shahzamani, K., Jahan Tigh, H. R., Malekshahian, S., & Esmaeil Lashgarian, H. (2018). Cytotoxic Effects of Coated Gold Nanoparticles on PC12 Cancer Cell. Galen Medical Journal, 7, e1110. https://doi.org/10.31661/gmj.v7i0.1110
Issue
Vol. 7 (2018): 2018
Section
Original Article

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