Cover Image

Chronotropic, Inotropic and Dromotropic Parameters of the Heart and Oxidative Stress in Rats Receiving High Doses of Fructose

Esmat Radmanesh, Mahin Dianat, Narges Atefipour

Background: Many risk factors, including nutritional ones, contribute to cardiovascular diseases (CVDs). Increased fructose consumption, for example, can lead to an increase in CVD risk factors, i.e. an increase in blood lipids and the development of insulin resistance. Materials and Methods: In the present study, Sprague Dawley rats were divided into two groups:  control group (free access to tap drinking water for seven weeks), and a group that received fructose 10% in drinking water for seven weeks, (n ═8 per each group). In all groups, before starting the test period and seven weeks after it, electrocardiogram was recorded by Power lab system. Unpaired t-test and two-way ANOVA were used for data analysis. Also, oxidative stress parameters were measured. Results: In the group received high doses of fructose, a significant reduction (P <0.05) was observed in the PR interval (P<0.001) and a significant increase (P<0.05) in the QTc interval. However, there was no significant change in the RR interval and the voltage of the QRS complex. A significant decrease in catalase, superoxide dismutase and glutathione peroxidase (P<0.05) and a significant increase (P<0.05) in malondialdehyde and lactate dehydrogenase were observed in the group that received fructose in comparison with the control group at the end of the experiment. Conclusion: According to our results, the chance of arrhythmias in the rats receiving high doses of fructose was possibly due to the increased oxidative stress in the healthy rats. [GMJ.2019;8:e1250]

 

Electrophysiological Parameters; Oxidative Stress; Rat; Fructose

Rosamond W, Flegal K, Friday G, Furie K. Go A, Greenlund K, et al. Heart disease and stroke statistics--2007 update: a report from the American Heart Association Statistics Committee and Stroke Statistics Subcommittee. Circulation. 2007; 115(5): e69-e171.

https://doi.org/10.1161/CIRCULATIONAHA.106.179918

Keating MT, Sanguinetti MC. Molecular and cellular mechanisms of cardiac arrhythmias. Cell. 2001; 104(4): 569-580.

https://doi.org/10.1016/S0092-8674(01)00243-4

Rippe JM , Angelopoulos TJ. Fructose-Containing Sugars and Cardiovascular Disease. Adv Nutr. 2015; 6(4):430-9.

https://doi.org/10.3945/an.114.008177

Semchyshyn HM. Fructation in vivo: detrimental and protective effects of fructose. Biomed Res Int. 2013; 9: Article ID 343914, 9 pages. http://dx.doi.org/10.1155/2013/343914.

https://doi.org/10.1155/2013/343914

Teff KL, Elliott SS, Tschop M, Kieffer TJ, Rader D, Heiman M, et al. Dietary fructose reduces circulating insulin and leptin, attenuates postprandial suppression of ghrelin, and increases triglycerides in women. J Clin Endocrinol Metab. 2004; 89(6):2963-2972.

https://doi.org/10.1210/jc.2003-031855

Le KA, Tappy L. Metabolic effects of fructose. Curr Opin Clin Nutr Metab Care. 2006; 9(4):469-475.

https://doi.org/10.1097/01.mco.0000232910.61612.4d

Dusting GJ, Triggle C. Are we over oxidized? Oxidative stress, cardiovascular Disease, and the future of intervention studies with antioxidants. Vasc Health Risk Manag. 2005; 1(2): 93-97.

https://doi.org/10.2147/vhrm.1.2.93.64080

Naito Y, Lee MC, Kato Y, Nagai R, Yonei Y. Oxidative Stress Markers. Anti-Aging Medicine. 2010; 7(5): 36-44.

https://doi.org/10.3793/jaam.7.36

Dokken BB. The Pathophysiology of Cardiovascular Disease and Diabetes: Beyond Blood Pressure and Lipids. Diabetes Spectrum. 2008; 21(3): 160-165.

https://doi.org/10.2337/diaspect.21.3.160

Girard A, Madani S, Boukortt F, Cherkaoui-Malki M, Belleville J, Prost J. ructose-enriched diet modifies antioxidant status and lipid metabolism in spontaneously hypertensive rats. Nutrition. 2006; 22: 758-766.

https://doi.org/10.1016/j.nut.2006.05.006

Rebolledo OR, Marra CA, Raschia A, Rodriguez S, Gagliardino JJ. Abdominal adipose tissue: Early metabolic dysfunction associated to insulin resistance and oxidative stress induced by an unbalanced diet. Horm. Metab. Res. 2008; 40: 794-800.

https://doi.org/10.1055/s-2008-1081502

Thirunavukkarasu V, Anuradha CV. Influence of alpha-lipoic acid on lipid peroxidation and antioxidant defence system in blood of insulin-resistant rats. Diabetes Obes. Metab. 2004; 6: 200-207.

https://doi.org/10.1111/j.1462-8902.2004.00332.x

Porto ML, Lírio LM, Dias AT, Batista AT, Campagnaro B P, et al. Increased oxidative stress and apoptosis in peripheral blood mononuclear cells of fructose-fed rats. Toxicol In Vitro. 2015; 29 (8): 1977-1981.

https://doi.org/10.1016/j.tiv.2015.08.006

Dhingra R, Sullivan L, Jacques PF, Wang TJ, Fox CS, Meigs JB, et al . Soft drink consumption and risk of developing cardiometabolic risk factors and the metabolic syndrome in middle-aged adults in the community. Circulation. 2007; 116: 480-8.

https://doi.org/10.1161/CIRCULATIONAHA.107.689935

Stanhope KL, Schwarz JM, Keim NL, Griffen SC, Bremer AA, Graham JL, Hatcher B, et al. Effects of consuming fructose- or glucose-sweetened beverages for 10 weeks on lipids, insulin sensitivity and adiposity. J Clin Invest. 2009; 119(5): 1322-1334. doi: 10.1172/JCI37385

https://doi.org/10.1172/JCI37385

Hyson D, Rutledge JC, Berglund L. Postprandial lipemia and cardiovascular disease. Curr Atheroscler Rep. 2003; 5:437-44.

https://doi.org/10.1007/s11883-003-0033-y

Dianat M, Veisi1 A, Ahangarpour A, Fathi Moghaddam H. The effect of hydro-alcoholic celery (Apiumgraveolens) leaf extract on cardiovascular parameters and lipid profile in animal model of hypertension induced by fructose. AJP. 2015; 5(3):203-9.

Lopes A, Vilela TC, Taschetto L, Vuolo F, Petronilho F, Dal-Pizzol F, et al. Evaluation of the effects of fructose on oxidative stress and inflammatory parameters in rat brain. Mol Neurobiol. 2014; 50(3):1124-30.

https://doi.org/10.1007/s12035-014-8676-y

Farah V, Elased KM, Chen Y, Key MP, Cunha TS, Irigoyen MC, et al. Nocturnal hypertension in mice consuming a high fructose diet. Auton Neurosci. 2006; 130: 41-50.

https://doi.org/10.1016/j.autneu.2006.05.006

Kamide K, Rakugi H, Higaki J, Okamura A, Nagai M, Moriguchi K, et al. The renin-angiotensin and adrenergic nervous system in cardiac hypertrophy in fructose-fed rats. Am J Hypertens. 2002; 15 (1 Pt 1): 66-71.

https://doi.org/10.1016/S0895-7061(01)02232-4

Axelsen LN, Lademann JB, Petersen JS, Holstein-Rathlou NH, Ploug T, Prats C, et al. Cardiac and metabolic changes in long-term high fructose-fat fed rats with severe obesity and extensive intramyocardial lipid accumulation. Am J Physiol Regul Integr Comp Physiol. 2010; 298(6):R1560-70.

https://doi.org/10.1152/ajpregu.00392.2009

Ghosian Moghadam M, Ansari I, Roghani M, Ghanem A, Mehdizade N. The Effect of Oral Administration of Hypericum Perforatumon Serum Glucose and Lipids, Hepatic Enzymes and Lipid Peroxidation in Streptozotocin-Induced Diabetic Rats. GMJ. 2017; 6(4): 319-29.

Refbacks

  • There are currently no refbacks.