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Gene and Biochemical Pathway Evaluation of Burns Injury via Protein-Protein Interaction Network Analysis

Majid Rezaei-Tavirani, Vahid Mansouri, Mostafa Rezaei Tavirani, Mohammad Rostami-Nejad, Davood Bashash, Mona Zamanian Azodi

Background: Severe burns injury can affect several vital systems in the body and can cause inflammation in organs such as the heart, liver, and kidney. Many inflammatory mediators and regulatory hormones related to burn injuries are recognized. In this study, the genes related to burn injury interacted via network analysis, and the central nodes were enriched through gene ontology (GO). Materials and Methods: Disease query of STRING database was used for data gathering, and the network was constructed using Cytoscape software version 3.6.0. After gene screening, the central nodes were enriched via GO analysis by ClueGO. The highlighted genes and pathways were clustered and analyzed in detail. Results: Among 1067 genes, 35 critical genes that are involved in the 14 highlighted biochemical pathways were recognized. Interpretation of the finding indicates that a number of central genes can be considered as potential biomarkers related to burn injury. Conclusion: Can we revise to “Burn injuries have features that are common to several  diseases and increases their risk. [GMJ.2019;8:e1257] 

Burn; Gene; Biomarker; Protein-Protein Interaction Network; Biochemical Pathway

Kaddoura I, Abu-Sittah G, Ibrahim A, Karamanoukian R, Papazian N. Burn injury: review of pathophysiology and therapeutic modalities in major burns. Ann Burns Fire Disasters. 2017;30(2):95.

Aulick LH, Wilmore DW, Mason Jr A, Pruitt Jr B. Influence of the burn wound on peripheral circulation in thermally injured patients. Am J Physiol. 1977;233(4):H520-26.

https://doi.org/10.1152/ajpheart.1977.233.4.H520

PMid:910969

Settle JA. Fluid therapy in burns. J R Soc Med. 1982;75(Suppl 1):6-11.

Herndon D, Abston S, Stein M. Increased thromboxane B2 levels in the plasma of burned and septic burned patients. Surg Gynecol Obstet.1984;159(3):210-3.

Morykwas MJ, David LR, Schneider AM, Whang C, Jennings DA, Canty C, et al. Use of subatmospheric pressure to prevent progression of partial-thickness burns in a swine model. J Burn Care Rehabil. 1999;20(suppl_1_pt_1):15-21.

https://doi.org/10.1097/00004630-199901001-00003

Qian W-J, Petritis BO, Kaushal A, Finnerty CC, Jeschke MG, Monroe ME, et al. Plasma proteome response to severe burn injury revealed by 18O-labeled "universal" reference-based quantitative proteomics. J Proteome Res. 2010;9(9):4779-89.

https://doi.org/10.1021/pr1005026

PMid:20698492 PMCid:PMC2945297

Seok J, Warren HS, Cuenca AG, Mindrinos MN, Baker HV, Xu W, et al. Genomic responses in mouse models poorly mimic human inflammatory diseases. Proc Natl Acad Sci U S A. 2013;110(9):3507-12.

https://doi.org/10.1073/pnas.1222878110

PMid:23401516 PMCid:PMC3587220

Safari-Alighiarloo N, Taghizadeh M, Rezaei-Tavirani M, Goliaei B, Peyvandi AA. Protein-protein interaction networks (PPI) and complex diseases. Gastroenterol Hepatol Bed Bench. 2014;7(1):17-31.

Maghvan PV, Rezaei-Tavirani M, Zali H, Nikzamir A, Abdi S, Khodadoostan M, et al. Network analysis of common genes related to esophageal, gastric, and colon cancers. Gastroenterol Hepatol Bed Bench. 2017;10(4):295-302.

Rezaei-Tavirani M, Rezaei-Tavirani S, Ahmadi N, Naderi N, Abdi S. Pancreatic adenocarcinoma protein-protein interaction network analysis. Gastroenterol Hepatol Bed Bench. 2017;10(Suppl1):S85-S92.

Safaei A, Tavirani MR, Azodi MZ, Lashay A, Mohammadi SF, Broumand MG, et al. Diabetic Retinopathy and Laser Therapy in Rats: A Protein-Protein Interaction Network Analysis. J Lasers Med Sci. 2017;8(Suppl 1):S20-S21.

https://doi.org/10.15171/jlms.2017.s4

PMid:29071030 PMCid:PMC5642173

Zali H, Rezaei Tavirani M. Meningioma protein-protein interaction network. Arch Iran Med. 2014;17(4):262-72.

Giorgio M, Migliaccio E, Orsini F, Paolucci D, Moroni M, Contursi C, et al. Electron transfer between cytochrome c and p66Shc generates reactive oxygen species that trigger mitochondrial apoptosis. Cell. 2005;122(2):221-33.

https://doi.org/10.1016/j.cell.2005.05.011

PMid:16051147

Henquin J-C, Ibrahim MM, Rahier J. Insulin, glucagon and somatostatin stores in the pancreas of subjects with type-2 diabetes and their lean and obese non-diabetic controls. Sci Reports. 2017;7(1):11015.

https://doi.org/10.1038/s41598-017-10296-z

PMid:28887444 PMCid:PMC5591190

Young CD, Anderson SM. Sugar and fat-that's where it's at: metabolic changes in tumors. Breast Cancer Res. 2008;10(1):202.

https://doi.org/10.1186/bcr1852

PMid:18304378 PMCid:PMC2374962

Colell A, Green D, Ricci J. Novel roles for GAPDH in cell death and carcinogenesis. Cell Death Differ. 2009;16(12):1573.

https://doi.org/10.1038/cdd.2009.137

PMid:19779498

Cohen P, Miyazaki M, Socci ND, Hagge-Greenberg A, Liedtke W, Soukas AA, et al. Role for stearoyl-CoA desaturase-1 in leptin-mediated weight loss. Science. 2002;297(5579):240-3.

https://doi.org/10.1126/science.1071527

PMid:12114623

Wadhwa R, Song S, Lee J-S, Yao Y, Wei Q, Ajani JA. Gastric cancer-molecular and clinical dimensions. Nat Rev Clin Oncol. 2013;10(11):643.

https://doi.org/10.1038/nrclinonc.2013.170

PMid:24061039 PMCid:PMC3927982

Milde-Langosch K. The Fos family of transcription factors and their role in tumourigenesis. Eur J Cancer. 2005;41(16):2449-61.

https://doi.org/10.1016/j.ejca.2005.08.008

PMid:16199154

Hollern D, Yuwanita I, Andrechek E. A mouse model with T58A mutations in Myc reduces the dependence on KRas mutations and has similarities to claudin-low human breast cancer. Oncogene. 2013;32(10):1296.

https://doi.org/10.1038/onc.2012.142

PMid:22525269

Akdis M, Burgler S, Crameri R, Eiwegger T, Fujita H, Gomez E, et al. Interleukins, from 1 to 37, and interferon-γ: receptors, functions, and roles in diseases. J Allergy Clin Immunol. 2011;127(3):701-21.

https://doi.org/10.1016/j.jaci.2010.11.050

PMid:21377040

Schuldiner M, Yanuka O, Itskovitz-Eldor J, Melton DA, Benvenisty N. Effects of eight growth factors on the differentiation of cells derived from human embryonic stem cells. Proc Natl Acad Sci U S A. 2000;97(21):11307-12.

https://doi.org/10.1073/pnas.97.21.11307

PMid:11027332 PMCid:PMC17196

Means AR, VanBerkum MF, Bagchi I, Lu KP, Rasmussen CD. Regulatory functions of calmodulin. Pharmacol Ther. 1991;50(2):255-70.

https://doi.org/10.1016/0163-7258(91)90017-G

Grieco L, Calzone L, Bernard-Pierrot I, Radvanyi F, Kahn-Perles B, Thieffry D. Integrative modelling of the influence of MAPK network on cancer cell fate decision. PLoS Comput Biol. 2013;9(10):e1003286.

https://doi.org/10.1371/journal.pcbi.1003286

PMid:24250280 PMCid:PMC3821540

Hockenbery D, Nuñez G, Milliman C, Schreiber RD, Korsmeyer SJ. Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature. 1990;348(6299):334.

https://doi.org/10.1038/348334a0

PMid:2250705

Porter AG, Jänicke RU. Emerging roles of caspase-3 in apoptosis. Cell Death Differ. 1999;6(2):99.

https://doi.org/10.1038/sj.cdd.4400476

PMid:10200555

Eljaiek R, Heylbroeck C, Dubois M-J. Albumin administration for fluid resuscitation in burn patients: A systematic review and meta-analysis. Burns. 2017;43(1):17-24.

https://doi.org/10.1016/j.burns.2016.08.001

PMid:27613476

Doig GS. Albumin may significantly increase mortality in burn patients: Re-analysis of a systematic review. Burns. 2017;43(2):449-50.

https://doi.org/10.1016/j.burns.2016.09.033

PMid:27884408

Wilmore DW, Aulick LH. Metabolic changes in burned patients. Surg Clin North Am. 1978;58(6):1173-87.

https://doi.org/10.1016/S0039-6109(16)41685-3

Lu X-M, Tompkins RG, Fischman A. Nitric oxide activates intradomain disulfide bond formation in the kinase loop of Akt1/PKBα after burn injury. Int J Mol Med. 2013;31(3):740-50.

https://doi.org/10.3892/ijmm.2013.1241

PMid:23314241 PMCid:PMC3597556

Luo G, Peng D, Zheng J, Chen X, Wu J, Elster E, et al. The role of NO in macrophage dysfunction at early stage after burn injury. Burns. 2005;31(2):138-44.

https://doi.org/10.1016/j.burns.2004.09.009

PMid:15683683

McCubrey JA, Steelman LS, Abrams SL, Lee JT, Chang F, Bertrand FE, et al. Roles of the RAF/MEK/ERK and PI3K/PTEN/AKT pathways in malignant transformation and drug resistance. Adv Enzyme Regul. 2006;46(1):249-79.

https://doi.org/10.1016/j.advenzreg.2006.01.004

PMid:16854453

Guo Y, Dickerson C, Chrest FJ, Adler WH, Munster AM, Winchurch RA. Increased levels of circulating interleukin 6 in burn patients. Clin Immunol Immunopathol. 1990;54(3):361-71.

https://doi.org/10.1016/0090-1229(90)90050-Z

Cannon JG, Friedberg JS, Gelfand JA, Tompkins RG, Burke JF, Dinarello CA. Circulating interleukin-1 beta and tumor necrosis factor-alpha concentrations after burn injury in humans. Crit Care Med. 1992;20(10):1414-9.

https://doi.org/10.1097/00003246-199210000-00009

PMid:1395662

Chollet-Martin S, Hernvann A, Lioret N, Lim S, Vaubourdolle M, Guechot J, et al. Cytokine response to burn injury: relationship with protein metabolism. J Trauma. 1994;36(5):624-8.

https://doi.org/10.1097/00005373-199405000-00004

PMid:8189461

Brown GL, Nanney LB, Griffen J, Cramer AB, Yancey JM, Curtsinger III LJ, et al. Enhancement of wound healing by topical treatment with epidermal growth factor. N Engl J Med. 1989;321(2):76-9.

https://doi.org/10.1056/NEJM198907133210203

PMid:2659995

Wilson Y, Goberdhan N, Dawson R, Smith J, Freedlander E, Mac Neil S. Investigation of the presence and role of calmodulin and other mitogens in human burn blister fluid. J Burn Care Rehabil. 1994;15(4):303-14.

https://doi.org/10.1097/00004630-199407000-00004

PMid:7929511

WJ Z, SF Z. Influence of oxidative stress on apoptosis and expression of bax and bcl-2 of enterocytes in burn rats with delayed resuscitation on the plateau. Zhonghua Shao Shang Za Zhi. 2009;25(4):289-93.

Aykac A, Karanlik B, Sehirli AO. Protective effect of silk fibroin in burn injury in rat model. Gene. 2018;641:287-91.

https://doi.org/10.1016/j.gene.2017.10.036

PMid:29037999

Dubois B, Epelbaum S, Nyasse F, Bakardjian H, Gagliardi G, Uspenskaya O, et al. Cognitive and neuroimaging features and brain β-amyloidosis in individuals at risk of Alzheimer's disease (INSIGHT-preAD): a longitudinal observational study. Lancet Neurol. 2018;17(4):335-46.

https://doi.org/10.1016/S1474-4422(18)30029-2

Stevenson B, Steelman J, Zimmerman LH, Bullard Z, Jurado L, Alsina G, et al. 936: Does Thrombin Dose Matter For Intraoperative Hemostasis? A Retrospective Case-controlled Study. Critical Care Med. 2018;46(1):451.

https://doi.org/10.1097/01.ccm.0000528943.37373.33

Lei JT, Shao J, Zhang J, Iglesia M, Chan DW, Cao J, et al. Abstract PD8-03: ESR1 gene fusions drive endocrine therapy resistance and metastasis in breast cancer. Cancer Res. 2018;78(4 Supplement):PD8-03-PD8-.

https://doi.org/10.1158/1538-7445.SABCS17-PD8-03

Morris RS, Schaffer BS, Lundy JB, Pidcoke HF, Chung KK, Darlington DN, et al. Immunopathological response to severe injury: platelet activation and the Th-17 immune response. Blood Coagul Fibrinolysis. 2018;29(1):48-54.

https://doi.org/10.1097/MBC.0000000000000665

PMid:28957941

Kang I, Chang MY, Wight TN, Frevert CW. Proteoglycans as Immunomodulators of the Innate Immune Response to Lung Infection. J Histochem Cytochem. 2018;66(4):241-259.

https://doi.org/10.1369/0022155417751880

PMid:29328866 PMCid:PMC5958380

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