Effects of Coenzyme Q10 and Diamond Nanoparticles on Ischemia-Reperfusion-Induced Testicular Damages in Rats

  • Masoumeh Masoumi 1. Department of Genetics, Faculty of biosciences, Islamic Azad University, North Tehran Branch, Tehran, Iran
  • Mitra Salehi 2. Department of Microbiology, Faculty of biosciences, Islamic Azad University, North Tehran Branch, Tehran, Iran
  • Seyed Abdolhamid Angaji 3. Department of Cell and Molecular Biology, Faculty of Biosciences, Kharazmi University, Tehran, Iran
  • Mehrdad Hashemi 4. Department of Genetics, Faculty of Advanced Science and Technology, Islamic Azad University, Tehran, Iran 5. Farhikhtegan Medical Convergence Sciences Research Center, Farhikhtegan Hospital Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
DOI: https://doi.org/10.31661/gmj.v10i0.2029
Keywords: Rats; CoQ10; Oxidative Stress; Testicle; Diamond Nanoparticles; Ischemia-Reperfusion

Abstract

Background: Ischemia-reperfusion (I/R) induced by testicular torsion can damage the testicles. In the present study, we assessed the effects of coenzyme Q10 (CoQ10) and diamond nanoparticles on sperm parameters in I/R testes in rats. Materials and Methods: Forty-eight Wistar adult male rats were divided into eight groups: healthy control (Ch), diamond nanoparticle healthy control group (Ch+Dia), CoQ10 healthy control group (Ch+Q10), diamond nanoparticles+CoQ10 healthy control group (Ch+Q10+Dia), torsion/detorsion group (Ct), the Ct group that received diamond nanoparticles (Ct+Dia), the Ct group that received CoQ10 (Ct+Q10), and Ct group that received diamond nanoparticles and CoQ10 (Ct+Q10+Dia). The rats were euthanized, and we collected the semen from the epididymal tissues to evaluate sperm viability, motility, concentration, and morphology parameters. Results: The I/R of the testicles significantly reduced sperm concentration, motility, viability, and altered sperm morphology in the rats. However, the administration of CoQ10 significantly improved sperm parameters in the rats with testicular I/R. Diamond nanoparticles decreased the sperm parameters; however, simultaneous administration of diamond nanoparticles and CoQ10 led to improved sperm parameters. Conclusion: CoQ10 potentially appeared to have protective effects against the long-term side-effects of I/R in testes in rats. Co-administration of diamond nanoparticles with CoQ10 significantly improved sperm parameters and greatly reduced the negative effects of diamond nanoparticles alone. Therefore, green synthesis of nanoparticles with the use of antioxidants such as CoQ10 is recommended. [GMJ.2021;10:e2029]

References

Asghari A, Akbari G, Meghdadi A, Mortazavi P. Effects of melatonin and metformin co-administration on testicular ischemia/reperfusion injury in rats. J Pediatr Urol. 2016;12(6):410.

https://doi.org/10.1016/j.jpurol.2016.06.017

PMid:27595505

Elshaari F, Elfageih R, Sheriff D. Testicular Torsion-Detorsion- Histological and Biochemical Changes in Rat Testis. J of Cytolog & Histology. 2012;3(1):136.

Lysiak JJ, Nguyen QA, Turner TT. Peptide and nonpeptide reactive oxygen scavengers provide partial rescue of the testis after torsion. J Androl. 2002;23(3):400-9.

Filho DW, Torres MA, Bordin AL, Crezcynski-Pasa TB, Boveris A. Spermatic cord torsion, reactive oxygen and nitrogen species and ischemia-reperfusion injury. Mol Aspects Med. 2004;25(1-2):199-210.

https://doi.org/10.1016/j.mam.2004.02.020

PMid:15051328

Etensel B, Ozkisacik S, Ozkara E, Karul A, Oztan O, Yazici M, Gürsoy H. Dexpanthenol attenuates lipid peroxidation and testicular damage at experimental ischemia and reperfusion injury. Pediatr Surg Int. 2007;23(2):177-81.

https://doi.org/10.1007/s00383-006-1781-x

PMid:16983563

Oldenburg O, Qin Q, Krieg T, Yang XM, Philipp S, Critz SD, Cohen MV, Downey JM. Bradykinin induces mitochondrial ROS generation via NO, cGMP, PKG, and mitoKATP channel opening and leads to cardioprotection. Am J Physiol Heart Circ Physiol. 2004;286(1): 468-76.

https://doi.org/10.1152/ajpheart.00360.2003

PMid:12958031

Bhagavan HN, Chopra RK. Coenzyme Q10: absorption, tissue uptake, metabolism and pharmacokinetics. Free Radic Res. 2006 ;40(5):445-53.

https://doi.org/10.1080/10715760600617843

PMid:16551570

Bentinger M, Brismar K, Dallner G. The antioxidant role of coenzyme Q. Mitochondrion. 2007;7:S41-S50.

https://doi.org/10.1016/j.mito.2007.02.006

PMid:17482888

Littarru GP, Tiano L, Belardinelli R, Watts GF. Coenzyme Q10, endothelial function, and cardiovascular disease. Biofactors. 2011;37(5):366-73.

https://doi.org/10.1002/biof.154

PMid:21674640

Sharifdini H, Parivar K, Hayati Rodbari N. Study of the effect of iron oxide nanoparticles on mouse testis development during the embryonic period in NMRI strain. NBR. 2017;4(3):215-25.

Ghafoori SM, Entezari M, Taghva A, Tayebi Z. Biosynthesis and evaluation of the characteristics of silver nanoparticles using Cassia fistula fruit aqueous extract and its antibacterial activity. ANSN. 2017;8(4):045019.

https://doi.org/10.1088/2043-6254/aa92bb

Yan L, Yang Y, Zhang W, Chen X. Advanced materials and nanotechnology for drug delivery. Adv Mater. 2014;26(31):5533-40.

https://doi.org/10.1002/adma.201305683

PMid:24449177

Agarwal A, Baskaran S, Parekh N, Cho CL, Henkel R, Vij S, Arafa M, Panner Selvam MK, Shah R. Male infertility. Lancet. 2021;397(10271):319-333.

https://doi.org/10.1016/S0140-6736(20)32667-2

Vaijayanthimala V, Lee DK, Kim SV, Yen A, Tsai N, Ho D, Chang HC, Shenderova O.. Nanodiamond-mediated drug delivery and imaging: challenges and opportunities. Expert Opin Drug Deliv. 2015;12(5):735-49.

https://doi.org/10.1517/17425247.2015.992412

PMid:25510332

World Medical Association; American Physiological Society. Guiding principles for research involving animals and human beings. Am J Physiol Regul Integr Comp Physiol. 2002;283(2): 281-3.

https://doi.org/10.1152/ajpregu.00279.2002

Yavari M, Talebi AR, Rezaei Zarchi S, Razavi S. Effects of Different Doses of Silver Nanoparticles on Sperm Parameters, Chromatin Structure and DNA Integrity in Mice. JCT, 2015; 6(2): 177-185.

Carden DL, Granger DN. Pathophysiology of ischaemia-reperfusion injury. J Pathol. 2000;190(3):255-66.

https://doi.org/10.1002/(SICI)1096-9896(200002)190:3<255::AID-PATH526>3.0.CO;2-6

Collard CD, Gelman S. Pathophysiology, clinical manifestations, and prevention of ischemia-reperfusion injury. Anesthesiology:. 2001;94(6):1133-8.

https://doi.org/10.1097/00000542-200106000-00030

PMid:11465607

Toyokuni S. Reactive oxygen species‐induced molecular damage and its application in pathology. Pathol Int. 1999;49(2):91-102.

https://doi.org/10.1046/j.1440-1827.1999.00829.x

PMid:10355961

Erol B, Bozlu M, Hanci V, Tokgoz H, Bektas S, Mungan G. Coenzyme Q10 treatment reduces lipid peroxidation, inducible and endothelial nitric oxide synthases, and germ cell-specific apoptosis in a rat model of testicular ischemia/reperfusion injury. Fertil Steril.. 2010;93(1):280-2.

https://doi.org/10.1016/j.fertnstert.2009.07.981

PMid:19683231

Feizi Y, Afzalpur M E, Abtahi-Eivary S. Effect of 2-weeks Coenzyme Q10 Supplementation on Malondialdehyde and Catalase Serum Levels Following Moderate and Severe Acute Resistance Training in Inactive Female Students. Horizon Med Sci. 2019; 25 (4) :256-269.

https://doi.org/10.32598/hms.25.4.256

Maulik N, Yoshida T, Engelman RM, Bagchi D, Otani H, Das DK. Dietary coenzyme Q10 supplement renders swine hearts resistant to ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol. 2000;278(4):H1084-H90.

https://doi.org/10.1152/ajpheart.2000.278.4.H1084

PMid:10749701

Belardinelli R, Tiano L, Littarru GP. Oxidative stress, endothelial function and coenzyme Q_ {10}. Biofactors. 2008;32(1-4):129-33.

https://doi.org/10.1002/biof.5520320115

PMid:19096108

Gromadzka-Ostrowska J, Dziendzikowska K, Lankoff A, Dobrzyńska M, Instanes C, Brunborg G, Gajowik A, Radzikowska J, Wojewódzka M, Kruszewski M. Silver nanoparticles effects on epididymal sperm in rats. Toxicol Lett. 2012;214(3):251-8.

https://doi.org/10.1016/j.toxlet.2012.08.028

PMid:22982066

Lafuente D, Garcia T, Blanco J, Sánchez D, Sirvent J, Domingo J, Gómez M. Effects of oral exposure to silver nanoparticles on the sperm of rats. Reprod Toxicol. 2016;60:133-9.

https://doi.org/10.1016/j.reprotox.2016.02.007

PMid:26900051

Adebayo O, Akinloye O, Adaramoye O. Cerium oxide nanoparticle elicits oxidative stress, endocrine imbalance and lowers sperm characteristics in testes of balb/c mice. Andrologia. 2018;50(3):e12920.

https://doi.org/10.1111/and.12920

PMid:29164652

Taylor U, Barchanski A, Garrels W, Klein S, Kues W, Barcikowski S, Rath D. Toxicity of gold nanoparticles on somatic and reproductive cells. Adv Exp Med Biol. 2012;733:125-33.

https://doi.org/10.1007/978-94-007-2555-3_12

PMid:22101718

Bai Y, Zhang Y, Zhang J, Mu Q, Zhang W, Butch ER, Snyder SE, Yan B. Repeated administrations of carbon nanotubes in male mice cause reversible testis damage without affecting fertility. Nat Nanotechnol. 2010;5(9):683-9.

https://doi.org/10.1038/nnano.2010.153

PMid:20693989 PMCid:PMC2934866

Duan J, Yu Y, Li Y, Wang J, Geng W, Jiang L, Li Q, Zhou X, Sun Z. Silica nanoparticles induce autophagy and endothelial dysfunction via the PI3K/Akt/mTOR signaling pathway. Int J Nanomedicine. 2014;9:5131-41.

https://doi.org/10.2147/IJN.S71074

PMid:25395850 PMCid:PMC4227623

Iravani S. Green synthesis of metal nanoparticles using plants. Green Chem. 2011;13(10):2638-50.

https://doi.org/10.1039/c1gc15386b

Published
2021-10-31
How to Cite
Masoumi, M., Salehi, M., Angaji, S. A., & Hashemi, M. (2021). Effects of Coenzyme Q10 and Diamond Nanoparticles on Ischemia-Reperfusion-Induced Testicular Damages in Rats. Galen Medical Journal, 10, e2029. https://doi.org/10.31661/gmj.v10i0.2029
Issue
Vol. 10 (2021): Continuous volume in progress
Section
Original Article

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).