Long Noncoding RNAs-LET Behave as a Noncoding Signature for Early-Onset Menarche and Late-Onset Menopause in Breast Cancer Patients

  • Farzaneh Darbeheshti 1. Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran 2. Breast Cancer Association (BrCA), Universal Scientific Education and Research Network (USERN), Tehran, Iran http://orcid.org/0000-0002-4957-9004
  • Hosein Mansoori 3. Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran 4. Breast Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran http://orcid.org/0000-0002-4796-8495
  • Rasoul Abdollahzadeh 1. Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Hassan Dastsooz 5. Dipartimento di Scienze della Vita e Biologia dei Sistemi, Università di Torino, Via Accademia Albertina, Torino, Italy
  • Abdolreza Daraei 6. Department of Genetics, School of Medicine, Babol University of Medical Sciences, Babol, Iran
  • Maral Mokhtari 7. Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
  • Hamzeh Salmani 1. Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Mostafa Davood Abadi Farahani 8. Department of Medical Genetics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
  • Sedigheh Tahmasebi 4. Breast Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  • Yaser Mansoori 9. Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran 10. Department of Medical Genetics, Fasa University of Medical Sciences, Fasa, Iran
Keywords: Expression, ceRNA, LncRNA-LET, Breast Cancer, Normal-Appearing Breast Tissue

Abstract

Background: Breast cancer (BC) as a major cause of cancer-related death in women shows a very complex molecular and clinical phenotype, which has reduced the effectiveness of medical interventions. Evidence suggests that long noncoding RNAs (lncRNAs) are responsible for an important part of this complexity. This study aims to assess the expression and clinical implication of lncRNA LET in the pathobiology of BC. Materials and Methods: Quantitative real-time polymerase chain reaction was used to measure the expression of lncRNA-LET in breast tumors and adjacent normal-appearing tissues from 4 BC patients, as well as normal mammary tissues. Moreover, a bioinformatics approach was applied to uncover the potential lncRNA-LET-mediated sponge regulatory network as LET/miRNA/mRNA crosstalk. Results: Our study revealed that lncRNA-LET was significantly down-expressed not only in breast tumors but also in normal appearing breast tissues samples from BC subjects compared with true normal breast tissues obtained from healthy women. The low level of lncRNA-LET was meaningfully associated with early-onset menarche (≤13 years) and late-onset menopause (≥50) in patients. Moreover, the bioinformatics analyses support that lncRNA-LET could function as a tumor suppressor miRNA sponge. Conclusion: The results indicate that normal appearing breast tissues can undergo tumor-related molecular changes. Furthermore, they reveal the potential role of the dysregulation in LET-mediated ceRNA network in the pathophysiology of BC.

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Published
2021-12-31
How to Cite
Darbeheshti, F., Mansoori, H., Abdollahzadeh, R., Dastsooz, H., Daraei, A., Mokhtari, M., Salmani, H., Davood Abadi Farahani, M., Tahmasebi, S., & Mansoori, Y. (2021). Long Noncoding RNAs-LET Behave as a Noncoding Signature for Early-Onset Menarche and Late-Onset Menopause in Breast Cancer Patients. Galen Medical Journal, 10, e2108. https://doi.org/10.31661/gmj.v10i0.2108
Section
Original Article