Evaluation of the Anti-Oxidative Effects of Monocyte Cells Treated with Bone Marrow Mesenchymal Stem Cells Supernatant on Experimental Colitis in BALB/c Mice
Abstract
Background: Alternate activation of monocytes could induce anti-inflammatory impacts. This study aimed to investigate whether monocyte cells treated with bone marrow mesenchymal stem cells supernatant (MSC-Sp) could improve anti-inflammatory responses as a cell transfer therapy for colitis. Materials and Methods: The induction of experimental colitis was done by acetic acid in four groups of male BALB/c mice, including the control colitis, treated-monocytes, non-treated-monocytes, and mesalazine groups. Following MSCs culture, the supernatant was harvested, and then 50% conditioned media, or negative control media was added to the monocytes for 24 h. After ten days, peritoneal injection of treated or non-treated-monocytes (105 cells/100µL) was performed in animals' relevant groups of colitis. Ten days later, the oxidative stress profile and histopathological evaluation of colon tissue were assessed. Results: Treated monocytes showed a significant improvement in the oxidative stress profile, namely myeloperoxidase (0.126±0.008), nitric oxide (0.153±0.01), and malondialdehyde (0.148±0.014) compared to the control colitis group (P<0.05). Also, histopathological results revealed that the rate of damage in the treated-monocytes group was less than in normal mice. Conclusion: Our study indicated that the treated monocytes had anti-oxidative potential in colitis mice and were usable as a complementary therapy. [GMJ.2021;10:e2131]References
Danese S, Fiorino G, Peyrin-Biroulet L. Positioning therapies in ulcerative colitis. Clin Gastroenterol Hepatol. 2020;18(6):1280-90.
https://doi.org/10.1016/j.cgh.2020.01.017
PMid:31982609
Corridoni D, Antanaviciute A, Gupta T, Fawkner-Corbett D, Aulicino A, Jagielowicz M, et al. Single-cell atlas of colonic CD8+ T cells in ulcerative colitis. Nat Med. 2020;26(9):1480-90.
https://doi.org/10.1038/s41591-020-1003-4
PMid:32747828
Nanki K, Fujii M, Shimokawa M, Matano M, Nishikori S, Date S, et al. Somatic inflammatory gene mutations in human ulcerative colitis epithelium. Nature. 2020;577(7789):254-9.
https://doi.org/10.1038/s41586-019-1844-5
PMid:31853059
Zhou X-L, Yang J, Qu X-J, Meng J, Miao R-R, Cui S-X. M10, a myricetin-3-ObD-Lactose sodium salt, prevents ulcerative colitis through inhibiting necroptosis in mice. Front Pharmacol. 2020;11:557312.
https://doi.org/10.3389/fphar.2020.557312
PMid:33041798 PMCid:PMC7517943
Wan S, Wu Q, Ji Y, Fu X, Wang Y. Promotion of the immunomodulatory properties and osteogenic differentiation of adipose‐derived mesenchymal stem cells in vitro by lentivirus‐mediated mir‐146a sponge expression. J Tissue Eng Regen Med. 2020;14(11):1581-91.
https://doi.org/10.1002/term.3113
PMid:32761798
Cao C, Tarlé S, Kaigler D. Characterization of the immunomodulatory properties of alveolar bone-derived mesenchymal stem cells. Stem Cell Res Ther. 2020;11(1):1-13.
https://doi.org/10.1186/s13287-020-01605-x
PMid:32138791 PMCid:PMC7059346
Liu H, Li R, Liu T, Yang L, Yin G, Xie Q. Immunomodulatory Effects of Mesenchymal Stem Cells and Mesenchymal Stem Cell-Derived Extracellular Vesicles in Rheumatoid Arthritis. Front Immunol. 2020;11:1912.
https://doi.org/10.3389/fimmu.2020.01912
PMid:32973792 PMCid:PMC7468450
Zhou Y, Fu B, Zheng X, Wang D, Zhao C, Qi Y, et al. Pathogenic T-cells and inflammatory monocytes incite inflammatory storms in severe COVID-19 patients. Natl Sci Rev. 2020;7(6):998-1002.
https://doi.org/10.1093/nsr/nwaa041
PMid:34676125 PMCid:PMC7108005
Esmaili Gourvarchin Galeh H, Meysam Abtahi Froushani S, Afzale Ahangaran N, Hadai SN. Effects of Educated Monocytes with Xenogeneic Mesenchymal Stem Cell-Derived Conditioned Medium in a Mouse Model of Chronic Asthma. Immunol Invest. 2018;47(5):504-20.
https://doi.org/10.1080/08820139.2018.1458108
PMid:29671652
Abtahi Froushani SM, Mashhouri S. The Effect of Mesenchymal Stem Cells Pulsed with 17 Beta-Estradiol in an Ameliorating Rat Model of Ulcerative Colitis. Zahedan Journal of Research in Medical Sciences. 2019.
https://doi.org/10.5812/zjrms.83762
Mashhouri S, Meysam Abtahi Froushani S, Asghar Tehrani A. Non-Adherent Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate Clinical Manifestations and Inflammation in an Experimental Model of Ulcerative Colitis in Rats.IJIMS. 2020;45(5):341-51.
Song N, Scholtemeijer M, Shah K. Mesenchymal Stem Cell Immunomodulation: Mechanisms and Therapeutic Potential. Trends Pharmacol Sci. 2020;41(9):653-64.
https://doi.org/10.1016/j.tips.2020.06.009
PMid:32709406 PMCid:PMC7751844
Harrell CR, Jovicic N, Djonov V, Arsenijevic N, Volarevic V. Mesenchymal Stem Cell-Derived Exosomes and Other Extracellular Vesicles as New Remedies in the Therapy of Inflammatory Diseases. Cells. 2019;8(12):1605.
https://doi.org/10.3390/cells8121605
PMid:31835680 PMCid:PMC6952783
Randhawa PK, Singh K, Singh N, Jaggi AS. A review on chemical-induced inflammatory bowel disease models in rodents. Korean J Physiol Pharmacol. 2014;18(4):279-88.
https://doi.org/10.4196/kjpp.2014.18.4.279
PMid:25177159 PMCid:PMC4146629
Piechota-Polanczyk A, Fichna J. Review article: the role of oxidative stress in pathogenesis and treatment of inflammatory bowel diseases. Naunyn Schmiedebergs Arch Pharmacol. 2014;387(7):605-20.
https://doi.org/10.1007/s00210-014-0985-1
PMid:24798211 PMCid:PMC4065336
Kurutas EB, Cetinkaya A, Bulbuloglu E, Kantarceken B. Effects of antioxidant therapy on leukocyte myeloperoxidase and Cu/Zn-superoxide dismutase and plasma malondialdehyde levels in experimental colitis. Mediators Inflamm. 2005;2005(6):390-4.
https://doi.org/10.1155/MI.2005.390
PMid:16489261 PMCid:PMC1533903
Kyurkchiev D, Bochev I, Ivanova-Todorova E, Mourdjeva M, Oreshkova T, Belemezova K, et al. Secretion of immunoregulatory cytokines by mesenchymal stem cells. World J Stem Cells. 2014;6(5):552-70.
https://doi.org/10.4252/wjsc.v6.i5.552
PMid:25426252 PMCid:PMC4178255
Yi T, Song SU. Immunomodulatory properties of mesenchymal stem cells and their therapeutic applications. Arch Pharm Res. 2012;35(2):213-21.
https://doi.org/10.1007/s12272-012-0202-z
PMid:22370776
Mittal M, Siddiqui MR, Tran K, Reddy SP, Malik AB. Reactive oxygen species in inflammation and tissue injury. Antioxid Redox Signal. 2014;20(7):1126-67.
https://doi.org/10.1089/ars.2012.5149
PMid:23991888 PMCid:PMC3929010
MacDonald ES, Barrett JG. The Potential of Mesenchymal Stem Cells to Treat Systemic Inflammation in Horses. Front Vet Sci. 2019;6:507.
https://doi.org/10.3389/fvets.2019.00507
PMid:32039250 PMCid:PMC6985200
Melief SM, Geutskens SB, Fibbe WE, Roelofs H. Multipotent stromal cells skew monocytes towards an anti-inflammatory interleukin-10-producing phenotype by production of interleukin-6. Haematologica. 2013;98(6):888-95.
https://doi.org/10.3324/haematol.2012.078055
PMid:23349310 PMCid:PMC3669444
Stavely R, Nurgali K. The emerging antioxidant paradigm of mesenchymal stem cell therapy. Stem Cells Transl Med. 2020;9(9):985-1006.
https://doi.org/10.1002/sctm.19-0446
PMid:32497410 PMCid:PMC7445024
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
