Effect of S14161 Small Molecule and Glaucium Flavum Extract on A549 Cancer Cells

  • Mahshad Kalantari 1. Department of Genetics, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  • Maliheh Entezari 1. Department of Genetics, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  • Milad Ashrafizadeh 2. Faculty of Engineering and Natural Sciences, Sabanci University, Orta Mahalle, Üniversite Caddesi, Orhanlı, Tuzla, Istanbul, Turkey 3. Sabanci University Nanotechnology Research and Application Center, Tuzla, Istanbul, Turkey
  • Abolfazl Movafagh 4. Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Kiavash Hushmandi 5. Department of Food Hygiene and Quality Control, Division of Epidemiology and Zoonoses, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Keywords: Lung Cancer, Glaucium Flavum, S14161 Small Molecule, Apoptosis, Gene Expression

Abstract

Background: Lung cancer is the fifth most common cancer in Iran. Due to the side effects of common cancer treatments, everyone has turned to herbal remedies and new treatments. This study aimed to compare the effect of S14161 small molecule and Glaucium flavum extract on the induction of apoptosis in A549 cancer cells. Materials and Methods: In this experimental study, the A549 cell line was treated with different concentrations of G. flavum and S14161 on days 1, 3, and 5. Also, half maximal inhibitory concentrations (IC 50) for both G. flavum and S14161 were measured. In addition, the quantitative real-time polymerase chain reaction (qRT-PCR) assay was used to investigate the effects of S14161 and G. flavum extract on the expressions level of Bax, Bad, P53, and Bcl2 genes. Results: Results showed that both the combination of S14161 and G. flavum extract resulted in cell death and reduced cancer cell viability. Nevertheless, the viability rate was greater by S14161, and this small molecule significantly increased the expression of Bax, P53, and Bad apoptotic genes and decreased the expression of the Bcl2 gene, which shows the induced apoptotic death and lethal effect of S14161 in comparison with G. flavum extract. Conclusion: Our study showed that S14161 had fewer IC50 and caused cell death by inhibiting the PI3K/AKT pathway, and G. flavum caused cancer cell death due to its alkaloid compounds. Therefore, both compounds are recommended as drug candidates for the treatment of lung cancer.

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Published
2021-12-31
How to Cite
Kalantari , M., Entezari, M., Ashrafizadeh, M., Movafagh, A., & Hushmandi, K. (2021). Effect of S14161 Small Molecule and Glaucium Flavum Extract on A549 Cancer Cells. Galen Medical Journal, 10, e2151. https://doi.org/10.31661/gmj.v10i0.2151