Dental Pulp Stem Cells Transplantation Improves Passive Avoidance Memory and Neuroinflammation in Trimethyltin-Induced Alzheimer’s Disease Rat Model

  • Samira Malekzadeh 1. Department of Biology, Fars Science and Research Branch, Islamic Azad University, Fars, Iran 2. Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
  • Mohammad Amin Edalatmanesh 2. Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
  • Davood Mehrabani 3. Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  • Mehrdad Shariati 4. Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran
Keywords: Stem Cells, Memory, Cognitive Dysfunction, Trimethyltin, Alzheimer Disease


Background: According to the increasing incidence of Alzheimer’s disease (AD), this study aimed to investigate the effect of dental pulp stem cells (DPSCs) transplantation on passive avoidance memory and neuroinflammation in trimethyltin (TMT)-induced AD rat model. Materials and Methods: In this experimental study, 18 male Wistar rats were randomly divided into three groups: the control that rats received 8 mg/kg TMT plus 0.5 ml phosphate buffered saline (PBS) and TMT+DPSCs (TMT + 1×106 cells/ml DPSC in 0.5 ml PBS) groups. Then, after one month, passive avoidance test was performed. Also measured the Nuclear Factor Kappa-β (NF-Kβ) serum level and the percentage of damaged neurons in the hippocampus were determined. Results: DPSCs transplantation showed significantly increased step-through latency to the dark compartment in comparison with control and TMT+PBS groups in 24 hours after shock. Also, time spent in the dark compartment of TMT+DPSCs significantly decreased compared to control and TMT+PBS groups in 24 and 48 hours after shock (P<0.05). Furthermore, DPSCs transplantation significantly decreased the NF-Kβ serum level and percentage of damaged pyramidal neurons of CA1 compared with TMT+PBS (P<0.05). Conclusion: DPSCs transplantation improved memory and learning, regulated NF-Kβ serum level, and decreased damage neurons of CA1 hippocampus in TMT-induced AD rat model.


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How to Cite
Malekzadeh, S., Edalatmanesh, M. A., Mehrabani, D., & Shariati, M. (2021). Dental Pulp Stem Cells Transplantation Improves Passive Avoidance Memory and Neuroinflammation in Trimethyltin-Induced Alzheimer’s Disease Rat Model. Galen Medical Journal, 10, e2254.