Serum Levels of Fatty Acid Binding Protein 5 Decreased in Squamous Cell Lung Carcinoma
AbstractBackground: The aberrant expression of Dickkopf-1 (DKK1), triose phosphate isomerase (TPI) and fatty acid binding-protein 5 (FABP5) in lung cancer tissues and their presence in secretome of lung cancer cell lines were reported. Here, serum levels of these molecules in lung cancer patients were compared to those in a healthy control group.Materials and Methods: The patients’ group consisted of 50 newly diagnosed male patients with a mean age of 65.3±11.5 years (27 squamous cell carcinoma (SCC), 7 adenocarcinoma, and 16 small cell carcinoma). Thirty eight apparently healthy men (mean aged 65.1±11.4) served as the control group. ELISA was employed for quantification.Results: Comparison between patients and controls revealed no significant difference, although FABP5 level was lower in patients (3.2 vs. 4.8; p=0.09). Analysis of each type of lung cancer with controls indicated that patients with SCC had significantly lower levels of FABP5 than in controls (2.7 vs. 4.8 ng/ml; P=0.03).Conclusion: Comparison between patients and controls revealed no significant difference, although FABP5 level was lower in patients (3.2 vs. 4.8; p=0.09). Analysis of each type of lung cancer with controls indicated that patients with SCC had significantly lower levels of FABP5 than in controls (2.7 vs. 4.8 ng/ml; P=0.03).[GMJ. 2014;3(4):222-7]
Gabrielson E. Worldwide trends in lung cancer pathology. Respirology. 2006;11(5):533-8. Epub 2006/08/19.
Masoompour SM, Yarmohammadi H, Rezaianzadeh A, Lankarani KB. Cancer incidence in southern Iran, 1998-2002: results of population-based cancer registry. Cancer epidemiology. 2011;35(5):e42-7. Epub 2011/08/16.
Merle P, Janicot H, Filaire M, Roux D, Bailly C, Vincent C, et al. Early CYFRA 21-1 variation predicts tumor response to chemotherapy and survival in locally advanced non-small cell lung cancer patients. The International journal of biological markers. 2004;19(4):310-5. Epub 2005/01/14.
Planque C, Kulasingam V, Smith CR, Reckamp K, Goodglick L, Diamandis EP. Identification of five candidate lung cancer biomarkers by proteomics analysis of conditioned media of four lung cancer cell lines. Molecular & cellular proteomics : MCP. 2009;8(12):2746-58. Epub 2009/09/25.
Menezes ME, Devine DJ, Shevde LA, Samant RS. Dickkopf1: a tumor suppressor or metastasis promoter? International journal of cancer Journal international du cancer. 2012;130(7):1477-83. Epub 2011/09/29.
Sheng SL, Huang G, Yu B, Qin WX. Clinical significance and prognostic value of serum Dickkopf-1 concentrations in patients with lung cancer. Clinical chemistry. 2009;55(9):1656-64. Epub 2009/07/25.
Lee HS, Lee HE, Park do J, Kim HH, Kim WH, Park KU. Clinical significance of serum and tissue Dickkopf-1 levels in patients with gastric cancer. Clinica chimica acta; international journal of clinical chemistry. 2012;413(21-22):1753-60. Epub 2012/07/17.
Hussain M, Rao M, Humphries AE, Hong JA, Liu F, Yang M, et al. Tobacco smoke induces polycomb-mediated repression of Dickkopf-1 in lung cancer cells. Cancer research. 2009;69(8):3570-8. Epub 2009/04/09.
Yamabuki T, Takano A, Hayama S, Ishikawa N, Kato T, Miyamoto M, et al. Dikkopf-1 as a novel serologic and prognostic biomarker for lung and esophageal carcinomas. Cancer research. 2007;67(6):2517-25. Epub 2007/03/17.
Mojtahedi Z, Safaei A, Yousefi Z, Ghaderi A. Immunoproteomics of HER2-positive and HER2-negative breast cancer patients with positive lymph nodes. Omics : a journal of integrative biology. 2011;15(6):409-18. Epub 2011/05/17.
Kim JE, Koo KH, Kim YH, Sohn J, Park YG. Identification of potential lung cancer biomarkers using an in vitro carcinogenesis model. Experimental & molecular medicine. 2008;40(6):709-20. Epub 2009/01/01.
Yousefi Z, Sarvari J, Nakamura K, Kuramitsu Y, Ghaderi A, Mojtahedi Z. Secretomic analysis of large cell lung cancer cell lines using two-dimensional gel electrophoresis coupled to mass spectrometry. Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society. 2012;50(3):368-74. Epub 2012/10/09.
Zhang XZ, Xiao ZF, Li C, Xiao ZQ, Yang F, Li DJ, et al. Triosephosphate isomerase and peroxiredoxin 6, two novel serum markers for human lung squamous cell carcinoma. Cancer science. 2009;100(12):2396-401. Epub 2009/09/10.
Madsen P, Rasmussen HH, Leffers H, Honore B, Celis JE. Molecular cloning and expression of a novel keratinocyte protein (psoriasis-associated fatty acid-binding protein [PA-FABP]) that is highly up-regulated in psoriatic skin and that shares similarity to fatty acid-binding proteins. The Journal of investigative dermatology. 1992;99(3):299-305. Epub 1992/09/01.
Masouye I, Hagens G, Van Kuppevelt TH, Madsen P, Saurat JH, Veerkamp JH, et al. Endothelial cells of the human microvasculature express epidermal fatty acid-binding protein. Circulation research. 1997;81(3):297-303. Epub 1997/09/01.
Fang LY, Wong TY, Chiang WF, Chen YL. Fatty-acid-binding protein 5 promotes cell proliferation and invasion in oral squamous cell carcinoma. Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology. 2010;39(4):342-8. Epub 2009/12/31.
Morgan E, Kannan-Thulasiraman P, Noy N. Involvement of Fatty Acid Binding Protein 5 and PPARbeta/delta in Prostate Cancer Cell Growth. PPAR research. 2010;2010. Epub 2010/09/18.
Buhrens RI, Amelung JT, Reymond MA, Beshay M. Protein expression in human non-small cell lung cancer: a systematic database. Pathobiology : journal of immunopathology, molecular and cellular biology. 2009;76(6):277-85. Epub 2009/12/04.
Yeung DC, Xu A, Tso AW, Chow WS, Wat NM, Fong CH, et al. Circulating levels of adipocyte and epidermal fatty acid-binding proteins in relation to nephropathy staging and macrovascular complications in type 2 diabetic patients. Diabetes care. 2009;32(1):132-4. Epub 2008/10/22.
Green RM, Gally F, Keeney JG, Alper S, Gao B, Han M, et al. Impact of cigarette smoke exposure on innate immunity: a Caenorhabditis elegans model. PloS one. 2009;4(8):e6860. Epub 2009/09/01.
Miller YE, Blatchford P, Hyun DS, Keith RL, Kennedy TC, Wolf H, et al. Bronchial epithelial Ki-67 index is related to histology, smoking, and gender, but not lung cancer or chronic obstructive pulmonary disease. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology. 2007;16(11):2425-31. Epub 2007/11/17.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).