Assessment of Toxoplasma Seropositivity in Children Suffering from Attention Deficit Hyperactivity Disorder

  • Shahla Afsharpaiman Health Research Center, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran
  • Mohammad Hossein Khosravi Students' Research Committee(SRC), Baqiyatallah University of Medical Sciences, Tehran, Iran
  • Mahdi Faridchehr Students’ Research Committee, Baqiyatallah University of Medical Sciences, Tehran, Iran
  • Mojtaba Komijani Students’ Research Committee, Baqiyatallah University of Medical Sciences, Tehran, Iran
  • Shokoofeh Radfar Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran
  • Susan Amirsalari The New Technology of Hearing Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
  • Mohammad Torkaman Department of pediatrics, Baqiyatallah University of Medical Sciences, Tehran, IR Iran
  • Shahnaz Shirbazoo Department of Parasitology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Keywords: Attention Deficit Hyperactivity Disorder, Toxoplasmosis, Toxoplasma Gondii, Children


Background: Toxoplasmosis, a protozoan infection caused by Toxoplasma gondii, is suggested to be a risk factor for many psychological disorders such as schizophrenia and depression. Attention deficit hyperactivity disorder (ADHD) is one of the most common psychiatric disorders among children. Previous studies have assessed the correlation between ADHD and infectious diseases like toxoplasmosis. So we aimed to investigate the possible correlation between Toxoplasma seropositivity and ADHD in children. Materials and Methods: In this cross-sectional study children with signs of ADHD were regarded as patients and underwent clinical assessments. Diagnosis of these patients was based on DSM-IV-TR system which was performed by a pediatric psychiatrist. Forty-eight Children without signs of ADHD or other psychic disorders were considered as control, and 48 patients were considered as case group. Parents were asked to answer a questionnaire including demographic, pregnancy and habitual questions. Blood samples were taken from all individuals and assessed for anti-Toxoplasma IgM and IgG antibodies. Results: Ninety-six male and female patients with a mean age of 8.12 years underwent analysis. Seropositivity rate for anti-T.gondii IgG antibody was 4.2% in the case and 2.1% in control individuals (P=0.92). Anti-T.gondii IgM antibodies were not found in control individuals while it was found in 2.1% of case individuals (P=0.74). There was no statistically significant association between seroprevalence of IgM (P=0.74) and IgG (P=0.92) antibodies and ADHD in study individuals. Conclusion: Our findings showed that the toxoplasmosis seropositivity has no significant difference between children with and without ADHD. Further studies are needed with a larger amount of individuals. [GMJ. 2016;5(4):188-93]


Brynska A, Tomaszewicz-Libudzic E, Wolanczyk T. Obsessive-compulsive disorder and acquired toxoplasmosis in two children. Eur Child Adolesc Psychiatry. 2001;10(3):200-4.

Brynska A, Wolanczyk T, Tomaszewicz-Libudzic C. OCD and acquired toxoplasmosis comorbmity in three children. Eur Psychiatry. 1997;12:222s.

Afsharpaiman S, Skandari A, Maryam ZJ, Radfar S, Shirbazoo S, Amirsalari S, et al. An assessment of Toxoplasmosis antibodies seropositivity in children suffering Autism. Tehran Univ Med J (TUMJ). 2014;72(2):106-12.

Silva D, Colvin L, Hagemann E, Bower C. Environmental risk factors by gender associated with attention-deficit/hyperactivity disorder. Pediatrics. 2014;133(1):e14-e22.

Sadock BJ, Sadock VA. Kaplan and Sadock’s synopsis of psychiatry: Behavioral sciences/clinical psychiatry: Lippincott Williams & Wilkins; 2011.

Polanczyk G, de Lima MS, Horta BL, Biederman J, Rohde LA. The worldwide prevalence of ADHD: a systematic review and metaregression analysis. Am J Psychiatry. 2007.

LaHoste G, Swanson J, Wigal S, Glabe C, Wigal T, King N, et al. Dopamine D4 receptor gene polymorphism is associated with attention deficit hyperactivity disorder. Mol Psychiatr. 1996;1(2):121-4.

Cabral CM, Tuladhar S, Dietrich HK, Nguyen E, MacDonald WR, Trivedi T, et al. Neurons are the primary target cell for the brain-tropic intracellular parasite Toxoplasma gondii. PLoS Pathog. 2016;12(2):e1005447.

Fekadu A, Shibre T, Cleare AJ. Toxoplasmosis as a cause for behaviour disorders-overview of evidence and mechanisms. Folia Parasitol. 2010;57(2):105.

Kliegman RM, Stanton B, Geme JS, Schor NF, Behrman RE. Nelson textbook of pediatrics: Elsevier Health Sciences; 2015.

Omar A, Bakar OC, Adam NF, Osman H, Osman A, Suleiman AH, et al. Seropositivity and Serointensity of Toxoplasma gondii Antibodies and DNA among Patients with Schizophrenia. Korean J Parasitol. 2015;53(1):29.

Torrey EF, Bartko JJ, Yolken RH. Toxoplasma gondii and other risk factors for schizophrenia: an update. Schizophr Bull. 2012:sbs043.

Benros ME, Waltoft BL, Nordentoft M, Østergaard SD, Eaton WW, Krogh J, et al. Autoimmune diseases and severe infections as risk factors for mood disorders: a nationwide study. JAMA psychiatry. 2013;70(8):812-20.

Dalman C, Allebeck P, Gunnell D, Harrison G, Kristensson K, Lewis G, et al. Infections

in the CNS during childhood and the risk of subsequent psychotic illness: a cohort study of more than one million Swedish subjects. Am J Psychiatry. 2008.

Fabiani S, Pinto B, Bruschi F. Toxoplasmosis and neuropsychiatric diseases: can serological studies establish a clear relationship? Neurol Sci. 2013;34(4):417-25.

Ziaali N, Fasihi Harandi, M.,Ahmadinejad, M. Study and comparison of latent toxoplasmosis in children with attention deficit/hyperactivity disorders and healthy children in Yazd city: Kerman University of Medical Sciences, Kerman, Iran; 2014.

Elgen I, Sommerfelt K, Markestad T. Population based, controlled study of behavioural problems and psychiatric disorders in low birthweight children at 11 years of age. Arch Dis Child Fetal Neonatal Ed.. 2002;87(2):F128-F32.

Ebadi M, Akhlaghi H, Zamani MM, Beheshti H, Abolhassani H, Ayadi A, et al. The Correlation Between Toxoplasma Gondii Infection and Schizophrenia: A Comparative Study With Family Members (Control Group). Scimetr. 2014;2(1).

Torrey EF, Bartko JJ, Lun Z-R, Yolken RH. Antibodies to Toxoplasma gondii in patients with schizophrenia: a meta-analysis. Schizophr bull. 2007;33(3):729-36.

Arling TA, Yolken RH, Lapidus M, Langenberg P, Dickerson FB, Zimmerman SA, et al. Toxoplasma gondii antibody titers and history of suicide attempts in patients with recurrent mood disorders. J Nerv Ment Dis. 2009;3(12):905-8.

Wang HL, Wang GH, Li QY, Shu C, Jiang MS, Guo Y. Prevalence of Toxoplasma infection in first episode schizophrenia and comparison between Toxoplasma seropositive and Toxoplasma seronegative schizophrenia. Acta Psychiatr Scand. 2006;114(1):40-8.

Daryani A, Sarvi S, Aarabi M, Mizani A, Ahmadpour E, Shokri A, et al. Seroprevalence of Toxoplasma gondii in the Iranian general population: A systematic review and meta-analysis. Acta Trop.. 2014;137:185-94.

Kerns KA, Mish SJ, Roberts J, Jagdis F. Neuropsychological Profile of Treated Children with Congenital Toxoplasmosis. Psychology. 2014;2014

How to Cite
Afsharpaiman, S., Khosravi, M. H., Faridchehr, M., Komijani, M., Radfar, S., Amirsalari, S., Torkaman, M., & Shirbazoo, S. (2016). Assessment of Toxoplasma Seropositivity in Children Suffering from Attention Deficit Hyperactivity Disorder. Galen Medical Journal, 5(4), 188-93.
Original Article