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Effects of Electro-Acupuncture and Acupotomy Dissolution on mRNA Expression of Center Pro-Opiomelanocortin Detected by In Situ Hybridization in Rats with Non-Specific Low Back Pain

Shapour Badiee Aval, Ali Khorsand, Seyed Javad Mojtabavi, Shima Rezaei Deloei, Guo Chang Qin

Background: Pro-opiomelanocortin (POMC) mainly exists in the pituitary gland, hypothalamus, and peripheral tissues and can relieve pain through its degradation product β-endorphin. Its mRNA expression quantity represents the level of gene expression of endorphin system. We aimed to determine the effects of electro-acupuncture and acupotomy dissolution on the mRNA expression of center POMC in rats with non-specific low back pain.Materials and Methods: This study was performed on 42 Sprague-Dawley rats in four groups of normal, model, electro-acupuncture, and acupotomy. The normal group did not receive any intervention, while non-specific low back pain was established in other groups. Then, the model group did not receive any treatment, electro-acupuncture and acupotomy groups were treated with electro-acupuncture therapy and acupotomy, respectively. Microscopic images of the slices, prepared from spinal dorsal horn and hypothalamus, were analyzed to evaluate the mRNA expression of center POMC. Results: Under light microscopy examination, the positive POMC mRNA cells of electro-acupuncture and acupotomy groups increased more than the model group, while its expression in the hypothalamus and spinal dorsal horn was less than the model group, but the difference was not significant (P<0.01). Conclusion: Electro-acupuncture and acupotomy could reduce POMC mRNA expression in spinal cord and increase it in the hypothalamus of rats with non-specific low back pain.[GMJ.2016;5(4):194-99]

Acupotomy; Electro-acupuncture; Non-specific Lower Back Pain; Pro-opiomelanocortin; mRNA Expression

Shaojie L, Lü S. Handbook of acupuncture in the treatment of musculoskeletal conditions. New York: Elsevier Health Sciences 2002.

Jinlin Qa. Study of Therapeutic Effects of cervicobrachialgia By Needle-Scalpel Therapy. Chin Acupunct Moxibust 1999;19:411.

Bin Z, Qian lY, Hai-tao W. Small needle-scalpel treatment of muscle injury histology and biomechanical study. Sport Sci 2004;24:28-31.

Changgeng Z. Immunocytochemical nerve. New York: Scientific Publishing House 1990.

Cabot PJ, Carter L, Gaiddon C, et al. Immune cell-derived beta-endorphin. Production, release, and control of inflammatory pain in rats. J Clin Invest 1997;100:142.

Xianhao X. Neuroimmunology. Beijing: Medical University Joint Publishing House 1993.

Mengyang Z. Removal of pituitary of rats brain 5-HT, norepinephrine and the effects of acupuncture analgesia. Acupunct Res 1984;2:129-32.

Draisci G, Iadarola MJ. Temporal analysis of increases in c-fos, preprodynorphin and preproenkephalin mRNAs in rat spinal cord. Mol Brain Res 1989;6(1):31-7.

Yi F, Fanrong L, Qiaolin T. Comparison of different needling on adjuvant arthritis in rats for analgesic effect of the hypothalamus. Shanghai J Acupunct 2005;24:38-41.

Canhuan Z. Electroacupuncture effect on rat adjuvant arthritis inflammation for partial opioid peptide gene expression. Jinan Univ 1997;18:158.

Jin C, Guangpu L, Chunyang Z. Hypothalamic β-EP and the expression of POMCmRNA after the effect of acupuncture analgesia in the role of acupuncture research. Chin J Integrat Med 2004;3:5-9.

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