Assessment of the Protective Impact of Vitamin E on Sex Hormones and Sperm Parameters of Formaldehyde-treated Male Rats: A Preliminary Investigation

  • Hassan Moladoust Department of Biochemistry and Medical physics, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
  • Ebrahim Nasiri Cellular and Molecular Research Center, Medical School, Guilan University of Medical Sciences, Rasht, Iran
  • Rouhollah Gazor Department of Anatomy, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
  • Tolue Mahdavi Department of Anatomy, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
  • Reza Ghorbani Department of Anatomy, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
  • Mohammad Rostampour Department of Physiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
Keywords: Formaldehyde, Vitamin E, Rat, Reproductive toxicity


Background: Lifestyle factors, including environmental and occupational exposure, have a key role in reproductive health status and may impact fertility. Formaldehyde (FA) is a suspected reproductive toxicant, which may cause significant adverse effects on the reproductive system. This study was aimed at detecting the impact of FA and the possible protective role of vitamin E on the male reproductive system in rats. Materials and Methods: Thirty-two adult male Wistar rats were randomly divided into four groups: control rats, rats treated with vehicle (corn-oil), rats treated with 10 mg/kg/day FA (FAt), and rats treated with FA plus 30 mg/kg/day vitamin E plus vehicle (FAt+ vitamin E) for two weeks. After treatment, sex hor­mone levels were examined using ELISA. Moreover the count, morphology, and motility of sperm, were observed. Results: The sperm count and the percentage of rapid progressive sperm were significantly decreased in rats in the FAt-treated group compared with those in the control and vehicle-treated groups (p<0.05). Vitamin E treatment significantly improved the parame­ters examined in the FAt+ vitamin E group (29.85±8.62 vs. 10.04±4.79 for sperm count and 60.50±5.67 vs. 42.19±8.02 for sperm motility). Moreover, serum follicle-stimulating hormone (FSH) levels mildly decreased in the FA exposure group, although the difference was not statis­tically significant (p>0.05). Conclusion: The findings of this study revealed that FA exposure had a negative impact on sperm parameters and some reproductive hormones in rats and vita­min E attenuated the deleterious impact of FA on the reproductive system of adult male rats. [GMJ.2017;6(4):330-7] DOI: 10.22086/gmj.v6i4.904


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How to Cite
Moladoust, H., Nasiri, E., Gazor, R., Mahdavi, T., Ghorbani, R., & Rostampour, M. (2017). Assessment of the Protective Impact of Vitamin E on Sex Hormones and Sperm Parameters of Formaldehyde-treated Male Rats: A Preliminary Investigation. Galen Medical Journal, 6(4), 330-337.
Original Article