Protective Effects of Red Onion (Allium cepa) Ethanolic Extract on Learning and Memory Impairments in Animal Models of Diabetes

  • Omid Reza Tamtaji Physiology Research Center, Kashan University of Medical Sciences, Kashan, Islamic Republic of Iran
  • Hossein Hosseinzadeh Pharmaceutical Research Center, Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Islamic Republic of Iran
  • Sayyed Alireza Talaei Physiology Research Center, Kashan University of Medical Sciences, Kashan, Islamic Republic of Iran
  • Mohammad Behnam Student Research Committee, Kashan University of Medical Sciences, Islamic Republic of Iran
  • Seyed Mahdi Takht Firoozeh Student Research Committee, Kashan University of Medical Sciences, Islamic Republic of Iran
  • Mohsen Taghizadeh Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, Islamic Republic of Iran
  • Reza Alipoor Student Research committee, Fasa University of Medical sciences, Fasa, Iran
Keywords: Onion, Memory, Learning, Streptozotocin, Rats


Background: Onion (Allium cepa) is a plant with antidiabetic and antioxidant properties. The aim of this study was to evaluate the protective effects of red onion ethanolic extract on learning and memory impairments in animal models of diabetes. Material and Methods: For induction of diabetes, streptozotocin (55 mg/kg) was injected intraperitoneally in male Wistar rats. Thirty-two male Wistar rats were randomly divided into 4 groups (n = 8). Diabetic rats were placed in 3 groups, including 2 groups treated with onion ethanolic extract (125 and 250 mg/kg/day) for 4 weeks and one diabetic control group. In addition, a fifth group was considered a health control group. Finally, learning and memory changes were evaluated through the Morris water maze and passive avoidance tests. Results: Our findings showed that escape latency and traveled distance were significantly increased in the diabetic control compared with the health control. The administration of onion extracts at 125 and 250 mg/kg significantly decreased the escape latency and traveled distance. In addition, the induction of diabetes caused a significant impairment in memory consolidation compared with healthy rats. However, animals that received extract at 125 mg/kg spent a longer time and traveled a greater distance in the target quarter compared with the diabetic control. Our data in the passive avoidance test also showed that diabetes reduced step-through latency in the animals. However, administration of onion ethanolic extract led to increased step-through latency in diabetic rats. Conclusions: Our findings show that oral administration of red onion ethanolic extract improves learning and memory performances impaired by streptozotocin in diabetic rats. [GMJ.2017;6(3):249-257] DOI:10.22086/gmj.v0i0.909


Malone JI, Hanna S, Saporta S, Mervis RF, Park CR, Chong L, et al. Hyperglycemia not hypoglycemia alters neuronal dendrites and impairs spatial memory. Pediatr Diabetes. 2008;9(6):531-9.

Forbes JM, Cooper ME. Mechanisms of diabetic complications. Physiol Rev. 2013;93(1):137-88.

Futamura A, Mori Y, Kawamura M. [Diabetes and Dementia]. Brain Nerve. 2015;67(6):725-32.

Huang RR, Jia BH, Xie L, Ma SH, Yin JJ, Sun ZB, et al. Spatial working memory impairment in primary onset middle‐age type 2 diabetes mellitus: An ethology and BOLD‐fMRI study. J Magn Reson Imaging. 2015.

Wang Y, Xu X-y, Feng C-h, Li Y-l, Ge X, Zong G-l, et al. Patients with type 2 diabetes exhibit cognitive impairment with changes of metabolite concentration in the left hippocampus. Metab Brain Dis. 2015:1-8.

Tonoli C, Heyman E, Roelands B, Pattyn N, Buyse L, Piacentini MF, et al. Type 1 Diabetes‐Associated Cognitive Decline: A Meta‐Analysis; an update of the current literature. J Diabetes. 2014; 6(6):499-513.

Gao Y, Xiao Y, Miao R, Zhao J, Zhang W, Huang G, et al. The characteristic of cognitive function in Type 2 diabetes mellitus. Diabetes Res Clin Pract. 2015; 109(2):299-305.

Kuhad A, Chopra K. Curcumin attenuates diabetic encephalopathy in rats: behavioral and biochemical evidences. Eur J Pharmacol. 2007;576(1):34-42.

Jabbarpour Z, Shahidi S, Saidijam M, Sarihi A, Hassanzadeh T, Esmaeili R. Effect of tempol on the passive avoidance and novel object recognition task in diabetic rats. Brain Res Bull. 2014;101:51-6.

Guo M, Mi J, Jiang QM, Xu JM, Tang YY, Tian G, et al. Metformin may produce antidepressant effects through improvement of cognitive function among depressed patients with diabetes mellitus. Clin Exp Pharmacol Physiol. 2014; 41(9):650-6.

Li R, Zang A, Zhang L, Zhang H, Zhao L, Qi Z, et al. Chrysin ameliorates diabetes-associated cognitive deficits in Wistar rats. Neurol Sci. 20143; 5(10):1527-32.

Liu Y, Tian X, Gou L, Sun L, Ling X, Yin X. Luteolin attenuates diabetes-associated cognitive decline in rats. Brain Res Bull. 2013;94:23-9.

Baluchnejadmojarad T, Roghani M. Chronic epigallocatechin-3-gallate ameliorates learning and memory deficits in diabetic rats via modulation of nitric oxide and oxidative stress. Behav Brain Res. 2011;224(2):305-10.

Bhutada P, Mundhada Y, Bansod K, Tawari S, Patil S, Dixit P, et al. Protection of cholinergic and antioxidant system contributes to the effect of berberine ameliorating memory dysfunction in rat model of streptozotocin-induced diabetes. Behav Brain Res. 2011;220(1):30-41.

Shaath NA, Flores FB. Egyptian onion oil. Developments in Food Science. 1998;40:443-53.

Machavarapu M, Sindiri MK, Vangalapati M. Optimization of Physico-chemical Parameters for the Extraction of Flavonoids and Phenolic Components from the Skin of Allium cepa. Optimization. 2013;2(7).

Pérez-Gregorio RM, García-Falcón MS, Simal-Gándara J, Rodrigues AS, Almeida DP. Identification and quantification of flavonoids in traditional cultivars of red and white onions at harvest. J Food Compost Anal. 2010;23(6):592-8.

Park S, Kim M-Y, Lee DH, Lee SH, Baik EJ, Moon C-H, et al. Methanolic extract of onion (Allium cepa) attenuates ischemia/hypoxia-induced apoptosis in cardiomyocytes via antioxidant effect. Eur J Nutr. 2009;48(4):235-42.

Hyun S-W, Jang M, Park SW, Kim EJ, Jung Y-S. Onion (Allium cepa) extract attenuates brain edema. Nutrition. 2013;29(1):244-9.

Kook S, Kim G-H, Choi K. The antidiabetic effect of onion and garlic in experimental diabetic rats: meta-analysis. J Med Food. 2009;12(3):552-60.

Takht Firoozeh SM TO, Farzin N,Taghizadeh M, Farahani G. Evaluation the effect of hydroalcoholic extract of Peganum harmala seed on weight and blood biochemical parameters in male Wistar rats. IEJM. 2015;17(2):124-8. [Persian]

Mahboubi M, Taghizadeh M, Talaei SA, Firozeh SMT, Rashidi AA, Tamtaji OR. Combined Administration of Melissa officinalis and Boswellia serrata Extracts in an Animal Model of Memory. Iran J Psychiatry Behav Sci. 2016; 10(3): e681.

Hwang IK, Lee CH, Yoo K-Y, Choi JH, Park OK, Lim SS, et al. Neuroprotective effects of onion extract and quercetin against ischemic neuronal damage in the gerbil hippocampus. J Med Food. 2009;12(5):990-5.

Shri R, Bora KS. Neuroprotective effect of methanolic extracts of Allium cepa on ischemia and reperfusion-induced cerebral injury. Fitoterapia. 2008;79(2):86-96.

Russell JW, Golovoy D, Vincent AM, Mahendru P, Olzmann JA, Mentzer A, et al. High glucose-induced oxidative stress and mitochondrial dysfunction in neurons. FASEB J. 2002;16(13):1738-48.

Vincent AM, McLean LL, Backus C, Feldman EL. Short-term hyperglycemia produces oxidative damage and apoptosis in neurons. FASEB J. 2005;19(6):638-40.

Moradkhani S, Salehi I, Abdolmaleki S, Komaki A. Effect of Calendula officinalis hydroalcoholic extract on passive avoidance learning and memory in streptozotocin-induced diabetic rats. Anc Sci Life. 2015; 34(3): 156–61.

El-Demerdash F, Yousef M, El-Naga NA. Biochemical study on the hypoglycemic effects of onion and garlic in alloxan-induced diabetic rats. Food Chem Toxicol. 2005;43(1):57-63.

Bang M, Kim H-A, Cho Y-J. Alterations in the blood glucose, serum lipids and renal oxidative stress in diabetic rats by supplementation of onion (Allium cepa. Linn). Nutr Res Pract. 2009;3(3):242-6.

Mahesh T, Menon VP. Quercetin allievates oxidative stress in streptozotocin‐induced diabetic rats. Phytother Res. 2004;18(2):123-7.

How to Cite
Tamtaji, O. R., Hosseinzadeh, H., Talaei, S. A., Behnam, M., Takht Firoozeh, S. M., Taghizadeh, M., & Alipoor, R. (2017). Protective Effects of Red Onion (Allium cepa) Ethanolic Extract on Learning and Memory Impairments in Animal Models of Diabetes. Galen Medical Journal, 6(3), 249-257.
Original Article